Abstract: Objective To investigate the effect of serum containing different concentrations of Traditional Chinese medicine Jinmaitong onβ-catenin、GSK-3βand P0 in Schwann cells cultured in high glucose medium. Methods Twenty-five male Sprague-Dawley rats were randomly treated with 5, 10, 15 and 20 times of Jinmaitong and distilled water. Schwann cells were divided into six groups, which are Control group, High glucose group, 5 times group, 10 times group, 15 times group, 20 times group. 72 hours later, the proliferative activity of SCs cells were detected by CCK, the mRNA and protein expression of β-catenin、P0 and GSK-3β was detectived by rt-PCR and Western blotting. Results High glucose medium could inhibit the proliferation of Schwann cells, down-regulate the expression of β-catenin and P0(P<0.01), and up-regulate the expression of GSK-3β(P<0.05) significantly. But Jinmaitong can invert the results (P<0.01, P<0.05). Conclusion High glucose medium will injure the proliferation of Schwann cells, But Jinmaitong can increase the proliferation activity of Schwann cells, and promotes the secretion of P0 Partially dependent on up-regulating the activity of β-catenin and down-regulating the activity of GSK-3β.

Key words: Jinmaitong Schwann cells Wnt/β-catenin Myelin Protein Zero Diabetic Peripheral Neuropathy