Abstract: Objective To study the effect of over-expression of KLF4 on the proliferation and migration ability of K562 cells. Methods The K562 cells with KLF4 over were obtained as experimental group, and paralleled with the vector control (K562-C1 cells) and blank K562 cell control. Real-time PCR were performed to detect the relative expression quantity of KLF4 mRNA of each group cells respectively;Western blot were performed to detect the level of KLF4 protein of each group cells respectively.The cell proliferation was tested by MTS assay.The migration ability of K562 cells was detected by Transwell. Results Compared with K562-C1 cells and blank K562 cells, the relative expression quantity of KLF4 mRNA of K562-KLF4 cells was significantly increased (P<0.05). The level of KLF4 protein of K562-KLF4 cells were significantly increased, which over-expression ratio was 77.78% (P<0.05).The proliferation ability and migration ability of the K562 cells with over-expressing KLF4 was inhibited significantly (P<0.05). Conclusions The over-expression of KLF4 could inhibit the proliferation and migration ability of K562 cells.

Key words: Key words: KLF4, K562 cell, cell proliferation, cell migration