Basic & Clinical Medicine ›› 2017, Vol. 37 ›› Issue (11): 1524-1528.

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RSUME promotes apoptosis of pituitary adenoma cell AtT-20 in mice


  • Received:2016-09-18 Revised:2016-12-26 Online:2017-11-05 Published:2017-11-01

Abstract: Objective Explore the expression and influence of RWD structure small ubiquitin modified enhancer (RSUME) and inhibitor of nuclear factor kappa B-alpha (IκB-α) and nuclear factor kappa B-1 (NF-κB1) in pituitary adenomas of mice. Methods Through RSUME small interfering RNA transfection pituitary adenomas, the AtT-20 cells in mice, the line of real-time fluorescent quantitative PCR (q-pcr) detect the level RSUME, IκB-α, NF-κB1 mRNA and WB (protein imprinting method) detect RSUME and the nucleoprotein of IκB-α, NF-κB1 changes in the level of protein, flow cytometry to detect cell apoptosis. Results In protein level, RSUME and IκB-α expression reduced(P<0.05), but NF-κB1 raised after transfection(P<0.05); The level of RSUME mRNA is obviously lower after transfection than before(P<0.05), with The level of IκB-α and NF-κB1 mRNA were no obvious change; Flow cytometry confirmed cell apoptosis rate increased significantly after transfection. Conclusions RSUME can promote apoptosis of pituitary adenoma cells in mice by NF-κB.

Key words: Key words:pituitary adenoma, cell apoptosis, RSUME, NF-κB, AtT-20

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