Abstract: Objective Explore the expression and influence of RWD structure small ubiquitin modified enhancer (RSUME) and inhibitor of nuclear factor kappa B-alpha (IκB-α) and nuclear factor kappa B-1 (NF-κB1) in pituitary adenomas of mice. Methods Through RSUME small interfering RNA transfection pituitary adenomas, the AtT-20 cells in mice, the line of real-time fluorescent quantitative PCR (q-pcr) detect the level RSUME, IκB-α, NF-κB1 mRNA and WB (protein imprinting method) detect RSUME and the nucleoprotein of IκB-α, NF-κB1 changes in the level of protein, flow cytometry to detect cell apoptosis. Results In protein level, RSUME and IκB-α expression reduced（P<0.05）, but NF-κB1 raised after transfection（P<0.05）; The level of RSUME mRNA is obviously lower after transfection than before（P<0.05）, with The level of IκB-α and NF-κB1 mRNA were no obvious change; Flow cytometry confirmed cell apoptosis rate increased significantly after transfection. Conclusions RSUME can promote apoptosis of pituitary adenoma cells in mice by NF-κB.

Key words: Key words:pituitary adenoma, cell apoptosis, RSUME, NF-κB, AtT-20