Basic & Clinical Medicine ›› 2016, Vol. 36 ›› Issue (9): 1268-1273.

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Effects of erythropoietin on endoplasmic reticulum stress-related proteins in the rat rhabdomyolysis induced acute kidney injury

1, LI Jian-dong 1,   

  • Received:2015-09-17 Revised:2016-01-03 Online:2016-09-05 Published:2016-08-30

Abstract: OBJECTIVE: To investigate whether erythropoietin (EPO) attenuates rhabdomyolysis-induced acute kidney injury by regulating endoplasmic reticulum stress associated proteins. METHODS: Healthy male SD rats were randomly divided into four group: control group (n=6), acute kidney injury group (AKI,n=18),AKI+EPO group (n=18), CN+EPO group (n=18), AKI group, AKI+EPO group and CN+EPO group based oninjection time is divided into three subgroups, 1, 6 and 24h groups of six rats. All animals were sacrificed on thire each time. Blood samples and kidney tissues were collected to evaluate blood urea nitrogen (BUN) , serum creatinine (SCr)and plasma Mb. The expression of GRP78 and CHOP was detected by immunohistochemistry and fluorescence quantitative RT-PCR. RESULTS: Compared with control group, significant increases in the levels of SCr, BUN and Mb were observed in AKI group and AKI+EPO group(P<0.05). The over-expression of AKI-induced GRP78 and CHOP was suppressed by EPO(P<0.05). CONCLUSION: AKI activates UPR in renal tubular epithelial cells. EPO has a protective effect on the kidneys with rhabdomyolysis-induced acute kidney injury, which may be related to the regulation of UPR-induced apoptosis.

Key words: erythropoietin, rhabdomyolysis, acute kidney injury, GRP78, CHOP