Basic & Clinical Medicine ›› 2016, Vol. 36 ›› Issue (6): 789-793.
Previous Articles Next Articles
Received:
Revised:
Online:
Published:
Contact:
Abstract: Objective To determine the effect of platelet-derived growth factor-C (PDGF-C) on ovarian cancer cells(SKOV3 and A2780)cultured in vitro. Methods The ovarian cancer cells(SKOV3 and A2780)were cultured in vitro. Cryopreserved SKOV3 and A2780 was cultured after water bath at 37 ℃. CCK8 assay was used to detect the effect of PDGF -C (0, 10, 20, 30 and 40 μg/L) on cell proliferation. Flow cytometry was used to detect cell phases. Transwell assay and cell scratch test were used to detect cell migration. Results PDGF-C significantly induced the proliferation of SKOV3 and A2780, and increased the percentage of the cells at S phase. Its effect on the SKOV3 and A2780 proliferation was found in a dose-dependent manner. PDGF-C of 20 μg/L promoted the SKOV3 proliferation at a summit, and PDGF-C of 20 μg/L promoted the A2780 proliferation at a summit. PDGF-C also promoted the migration ability of cultured SKOV3 and A2780. Conclusions PDGF-C promotes the proliferation and migration of cultured VECs and MSCs.
Key words: platelet-derived growth factor-C, A2780, SKOV3, cell proliferation, migration
CLC Number:
R711.75
0 / / Recommend
Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks
URL: http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/
http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/Y2016/V36/I6/789