Abstract: Objective To construct stably expressing TRIM22 HEK293T cell line and investigate the inhibitory function of TRIM22 to influenza A virus replication. Screen the IAV proteins which can interact with TRIM22. Methods Human TRIM22 gene was cloned onto pQC-XIP with FLAG tag using PCR. Then, the recombinant plasmid and pVSV-G, pGag-Pol were co-transfected into HEK293T cells for packaging retrovirus. Packaged retrovirus was collected and used to infect HEK293T cell, followed by screening with Puromycin. Finally, expression of TRIM22 was detected by Western blot. The inhibitory function of TRIM22 to influenza virus replication was detected with IAV-LUC. The interactions between IAV proteins and TRIM22 were detected by BiLC. Results Gene sequencing indicated that pQC-XIP-TRIM22 was successfully constructed. TRIM22 protein was detected in lysates of stable cell line. Contrast to stable cell line expressing EGFP, that expressing TRIM22 could inhibit the Influenza virus replication. TRIM22 can bind to NP. Conclusions Stable cell line expressing TRIM22 was successfully constructed. TRIM22 can inhibit the influenza virus and it can interact with NP.

Key words: TRIM22, retrovirus, HEK293T, influenza virus