Basic & Clinical Medicine ›› 2016, Vol. 36 ›› Issue (5): 678-681.

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α-Lipoic acid reduces TNF-α release of LPS-induced Raw264.7 cell


  • Received:2015-09-01 Revised:2016-03-16 Online:2016-05-05 Published:2016-04-26

Abstract: Objective To investigate the effect of α-lipoic acid on the release of TNF-α of LPS-induced Raw264.7 cell. Methods The Raw264.7 cell were pretreated with ALA or ALA and ERK /NF-κB inhibitors or activators for 2 h, and then stimulated with LPS (1 mg/L). The levels of tumor necrosis factor (TNF-α)in cultured supernatant were determined by ELISA kit.T-pERK,pERK and NF-κB were analyzed by Western blotting.Results ALA inhibited LPS-induced release of TNF-α(p<0.05),while the effect was reversible when the Raw264.7 cell were pretreated with ALA and ERK /NF-κB activators(p<0.05).ALA inhibited the phosphorytion of ERK and NF-κB (p<0.05),while the effect was strengthen when the Raw264.7 cell were pretreated with ALA and ERK /NF-κB inhibitors(p<0.05).Conclusions α-lipoic acid attenuates LPS-induced release of TNF-α by ERK and NF-κB signaling pathway.

Key words: α-lipoic acid, LPS, Raw264.7, TNF-α