Abstract: Objective To investigate whether livin expressed in gastric cancer SGC7901 cells could affect epithelial–mesenchymal transition (EMT). Methods SGC7901 cells were infected with livin shRNA lentivirus, stable transfection cells were selected. The invasion and migration ability of the stable transfection cells were detected by Transwell and Wound healing assay. The protein of E-cadherin and Vimentin were detected by Western blot. The activation of Akt signaling was detected by Western blot. Results Silencing livin in SGC7901 cells could inhibit the invasion and migration ability in vitro(P＜0.05). The protein of E-cadherin was significantly increased and vimentin was significantly decreased when livin was Silenced by livin shRNA(P＜0.05). Also, the activation of Akt signaling was significantly decreased(P＜0.05). Conclusion Livin expressed in gastric cancer cell promotes invasion and migration ability in vitro through induction of EMT by activation of AKT signaling.

Key words: Key words: shRNA, livin, EMT