Abstract: Objective To investigate the effect of specific integrin-linked kinase siRNA on proliferation and metastasis of human EJ bladder cancer cells and growth of transplanted bladder cancer. Methods Specific ILK siRNA plasmid and negative control plasmid were stably transfected into bladder cancer EJ cells.Then the effect of ILK siRNA on cytoskeleton was observed under laser confocal microscope.The expression of p-AKT，p-GSK-3β was evaluated by Western Blot.And the expression of p-AKT，p-GSK-3β of transfected cells was detected by immunofluorescence staining. RI co-localized with ILK in EJ cells by immunofluorescence experiments.The EJ cells was determined for proliferative activity by MTT and the distribution of cell cycle by flow cytometry.EJ cells stably expressing ILK siRNA were injected into nude mice.Morphology of tumor and lung tissue was observed with H&E staining.The expression of ILK，RI，NM23-H1 and E-Cadherin in tumor tissue was detected by immunohistochemistry. The change of p-AKT，p-GSK-3β and CD31 of transplanted bladder cancer was determined by immunofluorescence. Results ILK siRNA can inhibit the movement of EJ cells. Compared with control,EJ siRNA cells obviously decreased the expression of p-AKT，p-GSK-3β（P＜0.05）. The specific ILK siRNA can weaken the proliferating ability of EJ cells.ILK siRNA can inhibit the growth and metastasis of transplanted bladder cancer in nude mice. Conclusion The siRNA plasmid for ILK gene can inhibit EMT and decrease proliferation and metastasis ability significantly in EJ cells.

Key words: integrin-linked kinase, siRNA , EMT, EJ cells, transplanted tumor