Abstract: Objective To clone ESRP1 splicing variants and construct their lentivirus expression vector. To explore the effects of ESRP1 overexpression on MDA-MB-231 cell proliferation. Methods ESRP1 splicing variants were applified by PCR, in which the OVCAR3 cDNA was used as the template, and then the PCR products of ESRP1 splicing variants were ligated to Myc-tagged pCMV-Myc vector. ESRP1 splicing variants containing Myc-tag were cloned to lentivirus expression vector pLV-tTR/KRAB-Red. These lentivirus expression vectors containing Myc-tagged ESRP1 splicing variants were transfected into 293T cells, and lentivirus were made. MDA-MB-231 cells were infected with lentivirus, the cell proliferation was determined by MTT, and PARP cleavage was examined. Results The lentivirus expression vectors containing Myc-tagged ESRP1 splicing variants were constructed successfully, and the lentivirus were produced. ESRP1 overexpression could inhibit MDA-MB-231 cell proliferation and induce PARP cleavage in MDA-MB-231 cells. Conclusions The lentivirus expression vectors containing Myc-tagged ESRP1 splicing variants were constructed successfully. ESRP1 overexpression could inhibit MDA-MB-231 cell proliferation, and this may be correlated with cell death.

Key words: ESRP1, Breast cancer, Cell growth, Alternative splicing