Abstract: Objective To investigate the effect and mechanism of salidroside on cell cycle and proliferation via ERK signal pathway in mouse marrow mesenchymal stem cells. Methods The experiment including control group (D-MEM/F-12 complete medium), different concentration of salidroside induced group (5~100 μg/mL) and blocked group (30 μg/L PD98059 + 5~100 μg/mL). The cell cycle, inhibition rates and the expression levels of cell cycle-related proteins were detected by flow cytometry, CCK8 method and western blot respectively. Results The cell proliferation were inhited in 10 μg/mL induced group and blocked group, meanwhile the inhibition rates in 5 μg/mL (P<0.01), 25 μg/mL (P<0.05) and 100 μg/mL (P<0.05) induced groups were significantly higher than those in blocked groups. The G0/G1 phase cell rate in 25 μg/mL induced group and blocked group were both increased. The expression of CyclinA and CyclinD1 protein were downregulated, whereas p21 protein expression was upregulated in both induced group and blocked group to compared with the control group. The expression of CyclinA protein was downregulated in blocked group to compared with the induced group, the expression of CyclinD1 protein was downregulated in 50 and 100 μg/mL induced groups to compared with blocked group, whereas the expression of p21 protein in induced group was extremely higher than blocked group. Conclusions Salidroside could inhibit MSCs proliferation and affect cell cycle via regulating the expression of cell cycle-related proteins.

Key words: Salidroside, MSCs, Cell cycle, ERK signal pathway