Basic & Clinical Medicine ›› 2015, Vol. 35 ›› Issue (8): 1067-1071.

Previous Articles     Next Articles

Clinical significance and correlation of Yes-associated protein and β-catenin expression in gastric cancer

  

  • Received:2014-12-18 Revised:2015-03-26 Online:2015-08-05 Published:2015-07-15

Abstract: Objective To investigate the expressions and correlation of YAP andβ-catenin in the gastric carcinoma(GC) tissues and the paired normal gastric tissues. Methods Fluorescence quantitative PCR(RT-qPCR), immunocytochemistry staining and Western blotting were used to detect the mRNA and protein expressions of YAP and β-catenin in GC tissues and the paired normal gastric tissues,The correlations between the expressions and the clinicopathological characteristics of GC were analyzed statistically. Results The positive expression of YAP and β-catenin were respectively 72% (36/50) and 64% (32/50), and the positive expression of paired normal gastric tissues were 14% (7/50) and 20% (10/50), P<0.05; The mRNA and protein levels of YAP and β-catenin in GC tissues were higher than those in the paired normal tissues(P<0.05).Furthermore, the expression was obviously correlated to cell differentiation, lymph node metastases and clinical stage of GC( P< 0.05). The significantly positive correlation was found between the expressions of YAP and β-catenin in GC tissues(r=0.72, P<0.05). Conclusion The high expression of YAP and β-catenin coexist in GC tissues. It may be related to malignant transformation and abnormal proliferation of cells, which facilitates the occurrence and development of GC.

Key words: stomach neoplasm, Yes-associated protein, β-catenin, signal transduction pathway

CLC Number: