Abstract: Objective To investigate the expression and clinical significance of lysyl oxidase(Lox) and hypoxia inducible factor-1α(HIF-1α) in non-small cell lung cancer(NSCLC).Methods 41cases of NSCLC tissue specimen were collected from clinical,by immunohistochemical (IHC) detecting the expression of LOX and HIF-1αprotein in NSCLC; Protein expression of HIF-1α and LOX of NSCLC tissus, corresponding paraneoplastic tissues and normal lung tissue was examined through western blot. Results Immunohistochemistry staining results showed that LOX protein mainly expressed in the cytoplasm, HIF-1αprotein in the nucleus. The positive expression rate of LOX or HIF-1αin NSCLC tissues was significantly higher than that of paired normal tissues (P <0.05); The high expression of LOX protein is remarkably related with pathological type ,tumor differentiation, lymph node metastasis and TNM stage(P <0.05); The high expression of HIF-1αprotein is remarkably related with pathological type ,tumor size, lymph node metastasis and TNM stage(P <0.05); The LOX protein had significant positive correlation with the HIF-1α protein expression in NSCLC(P<0.05).Western blot results showed that HIF-1αor LOX protein expression levels in NSCLC was significantly higher than that of paired paraneoplastic tissues and normal tissues((P<0.05).Conclusions LOX or HIF-1αabnormal expression is probably relevant to occurrence and development of NSCLC, and higher expression of them may act in synergy to promote the invasion and metastasis of NSCLC.

Key words: Key words:LOX, HIF-1α, non-small cell lung cancer