Basic & Clinical Medicine ›› 2015, Vol. 35 ›› Issue (5): 637-641.

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Enhancement of Thermal Damage to EGFR-positive U251 Cancer Cells by Aptamer Modified Magnetic Nanoparticle


  • Received:2014-12-25 Revised:2015-03-13 Online:2015-05-05 Published:2015-04-28
  • Contact: Xian-da YANG

Abstract: Objective To develop a novel EGFR aptamer-modified magnetic nanoparticle (Apt-NP) for enhancing the targeted thermal damage to EGFR-positive U251 cancer cells. Method EGFR aptamer was implanted onto nanoparticles via biotin-streptavidin reaction. Apt-NP was characterized by dynamic light scattering (DLS) assay. The binding of aptamer to target cell was evaluated by flow cytometry. The attachment of Apt-NP to target cell was evaluated by Prussian blue staining. Thermal damage under alternative magnetic field was measured by detecting the release of LDH. Results The average size of Apt-NP was 574 nm. Apt-NP could bind with the EGFR-positive U251 tumor cells but not the MDA-MB-231 cell. Importantly,Apt-NP significantly enhanced the thermal damage to EGFR-positive U251 tumor cells but not that to MDA-MB-231 cells. Conclusions Aptamer-modified nanoparticles may have potential utility in tumor targeted thermal therapies.

Key words: Key words: aptamers, thermal therapy, EGFR, Glioblastomas

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