Basic & Clinical Medicine ›› 2015, Vol. 35 ›› Issue (5): 632-636.

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Validation of intergenic long non-coding RNA: T-UCRs for their transcriptional activities


  • Received:2015-01-23 Revised:2015-03-13 Online:2015-05-05 Published:2015-04-28
  • Contact: Xiao-zhong PENG

Abstract: Objective Exploration of the embryonic development related transcriptional activities of a specific class of lncRNA, T-UCR (Transcribed Ultra-conserved Regions), in the brain. Methods UCSC Genome Browser (NCBI37/mm9) RNA-seq, Coding Potential Assessment Tool (CPAT) and Coding Potential Calculator (CPC), RT-PCR and Real-time PCR were employed to identify T-UCR, to predict coding potential, to display the spatio-temporal patterns in different mouse adult tissues and different mouse development stages of embryonic brain, respectively. Results The results show that there are 15 intergenic T-UCRs expressed in mouse embryonic brains, which may function as long noncoding RNAs. T-uc.62 is prominently expressed in mouse embryonic brains, rather than other adult tissues. Conclusion The intergenic T-UCR can be transcribed to long noncoding RNAs, and T-uc.62 is expressed mainly in mouse embryonic brains, which infers that T-uc.62 may be important in brain development.

Key words: UCR, mouse brain development, T-uc.62

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