Abstract: Objective This research adopt the histone acetylation inhibitor Largazole to treat on human umbilical cord mesenchymal stem cells (hUCMSCs), and discuss the role and molecular mechanism of anti-aging. Methods hUCMSCs were isolated from human Umbilical cords after trypsin digestion, and determined by Flow cytometry analysis. they were cultured with different concentrations of largazole to find the fine cell proliferative concentrations. Passage 4, 8, 12, and 16 cells were subjected to real-time PCR for the change of stem cell gene expression on largazole group. We investigated the effect of largazole, against the histone H3 lysine 9 or 14 (H3K9/K14) acetylation of the gene (TERT, OCT4 and OPN) by CHIP assays. Results hUCMSCs can undergo spontaneous differentiation and aging during expansion. Low concentrate largazole can promote hUCMSCs proliferation and suppresse its spontaneous osteogenic differentiation. Largazole modulates histone H3 acetylation in the TERT, OCT4 and OPN genes; Conclusion our results indicate that histone H3 acetylation, which can be modulated by Largazole, plays a key role in regulating hUCMSCs biological characteristics.

Key words: stem cells, proliferation, biological characteristics