Abstract: Objective To determine the effects of EGCG on lipopolysaccharide (LPS) -induced neuroinflammation and further investigate the role of neuroprotection mediated by EGCG. Method Primary cultures of rat gliacyte were used as an in vitro model to examine the effects of EGCG on LPS-induced neuronal damage. The intracellular Glu and γ-GABA were quantified via HPLC.Then the protein levels of TNF-α,IL-1βand IL-8 were determined by ELISA and western blot assay. Result Compared with the control group, LPS apparently induced the production of intracellular ROS (P<0.05) and released the TNF-α, IL-1β and IL-8 in the primary cultures supernatant (P<0.05). Compared with the LPS group,EGCG significantly attenuated the release of TNF-α, IL-1β and IL-8(P<0.05) and the level of iNOS protein(P<0.05). Result Compared with the control group, LPS apparently induced the production of intracellular ROS(P<0.05) and released the TNF-α,IL-1β and IL-8 in the primary cultures supernatant (P<0.05).Compared with the LPS group, EGCG significantly attenuated the release of TNF-α, IL-1β and IL-8 (P<0.05) and the level of iNOS protein(P<0.05),and rugulated the concentration of Glu/γ-GABA (P<0.05).Conclusion EGCG is effective in protecting against LPS-induced neuroinflammation by anti-inflammatory effects and regulating extracellular Amino acid levels.

Key words: Key words: EGCG, LPS, Neuroinflammation, Amino acid, Arotection effect