Basic & Clinical Medicine ›› 2015, Vol. 35 ›› Issue (2): 187-190.
Previous Articles Next Articles
Received:
Revised:
Online:
Published:
Contact:
Abstract: Objective To investigate collagen metabolism modulation of Intermedin1-53 (IMD1-53) on angiotensin Ⅱ (Ang Ⅱ)-induced rat cardiac fibroblasts. Metheds Cultured SD neonatal rat cardiac fibroblasts, and divided them into control group, AngⅡ + different concentrations IMD1-53 groups.Ⅰand Ⅲ collagen expression in cardiac fibroblasts were measured by Westem blot, IMD1-53 receptor - like receptor (calcitonin receptor-like receptor, CRLR) and transforming growth factor-β (TGF-β) mRNA expression by SYBR Green Ⅰ real-time PCR. Results IMD1-53 significantly inhibited AngII-induced collagen synthesis in cardiac fibroblasts, and this effect was more obvious with the increasment of IMD1-53( P < 0.01,P < 0.05). Similar phenomenon took place on TGF-β expression in cardiac fibroblasts( P < 0.05). Conclusion IMD1-53 inhibited collagen synthetic in cardiac fibrosis induced by AngⅡ, downed TGF-β expression. These might relate to IMD1-53 anti myocardial fibrosis.
Key words: Intermedin1-53, cardiac fibroblast, angiotensin Ⅱ, transforming growth factor-β
CLC Number:
R331.3
0 / / Recommend
Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks
URL: http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/
http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/Y2015/V35/I2/187