Abstract: To identify the anti-HBV efficiency of AAV-IFN by infecting HepG2.2.15 cell line, and to develop a new strategy of curing hepatitis B. Method Firstly,recombinant vector was packaged into AAV by three plasmids co-transfection method; then AAV-IFNα infected HepG2.2.15 cell line, and samples were collected on the1st, 3rd,6th and 9th day; thirdly, The time-course of HBV DNA,HBsAg and HBeAg were detected by qPCR and ELISA. The proliferation of HepG2.2.15 cells detected by MTT. Result Recombinant AAV-IFN can significantly inhibit HBV replication and expression in vitro because of the proliferation of HepG2.2.15 cells inhibited by AAV6-IFNα（P＜0.05）. Conclusion AAV-IFN is a potential antiviral therapeuticsfor curing hepatitis B.

Key words: Key words：Hepatitis B virus (HBV), Adeno-associated virus (AVV), Interfron (IFN)