Basic & Clinical Medicine ›› 2015, Vol. 35 ›› Issue (1): 65-68.
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Abstract: Objective To investigate the effects of cyclooxygenase-2 inhibitor celecoxib on proliferation, invasion and migration of human pancreatic cancer cell line PANC-1 and then determine the optimal concentration of celecoxib and the most suitable application time. Methods Human pancreatic cancer cell line PANC-1 was treated with diverse concentrations of celecoxib (20、60、100μmol/L) for different durations (24、48、72h). Cell proliferation, invasion and migration capabilities were measured by MTT colorimetry, Transwell invasion assay, and scratch assay respectively. Results The proliferation capability of PANC-1 cell was reduced by celecoxib in a concentration- and time-dependent manner (P<0.05). In addition, the invasion and migration capabilities were decreased by celecoxib in a concentration-dependent manner (P<0.01). Conclusions Celecoxib attenuates the proliferation of human pancreatic cancer cell line PANC-1 in a concentration- and time-dependent manner. Celecoxib attenuates the invasion and migration in a concentration-dependent manner.
Key words: Pancreatic cancer, celecoxib, proliferation, invasion, migration
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URL: http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/
http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/Y2015/V35/I1/65