Abstract: Objective To investigate the effect of miR-30a on human osteosarcoma cell 143B migration,invasion and cell viability. Methods 143B cells were infected or transfected with recombinant adenovirus miR-30a (Ad-miR30a) and miR-30a inhibitor, respectively. Wound healing assay was performed to detect the cell healing ability(P<0.05). Cell migration and invasion ability were determined by Transwell assay(P<0.05).The cell viability was analyzed by MTT assay(P<0.01). Real-time quantitative PCR was performed to analyze the expression of RUNX2 mRNA level and confirmed the adenovirus miR-30a which would express in 143B cells.The expression of RUNX2 at protein level was analyzed by Western blot. miR-30a target to RUNX2 was verified by luciferase reported gene assay. Results The ability of migration and invasion was suppressed in osteosarcoma cell 143B by overexpression miR-30a,and the cell viability was also decreased .After the endogenous miR-30a was inhibited, the cell motility and invasion were enhanced, the cell viability was promoted.The RUNX2 protein was decreased after overexpression miR-30a compared with control group in 143B cell. The luciferase activity of RUNX2 was decreased by adding miR-30a.Conclusions 143B cell migration, invasion and viability were suppressed by miR-30a,and this process might be achieved via suppressing RUNX2 protein expression.

Key words: miR-30a, human osteosarcoma cell line, RUNX2