Abstract: Objecttive To study the effect of microRNA-34c on the development of hepatocellular carcinoma. Methods Expressions of microRNA-34c and c-Met protein in clinical hepatocellular carcinoma tissue and para-carcinoma tissue, c-Met mRNA and protein in HepG2.2.15 cells transfected with microRNA-34c, P53 protein in HepG2.2.15 cells transfected with microRNA-34c or c-Met siRNA were detected by qPCR and Western blot respectively. Xenograft nude mice modle of liver cancer was established and the volume of tumors was measured. Results MicroRNA-34c expression is significantly decreased in the hepatocellular carcinoma tissue compared with para-carcinoma tissue（P<0.01）,while the c-Met expression is inverse.MicroRNA-34c can inbit c-Met expression in HepG2.2.15 cells（P<0.01）.Downregulation of c-Met ehanced P53 activity（P<0.05）.MicroRNA-34c inhibited the growth of liver cancer in xenograft nude mice modle（P<0.05）. Conclusion MicroRNA-34c may play a role in suppressing the development of hepatocellular carcinoma by downregulating c-Met.

Key words: hepatocellular carcinoma, microRNA-34c, c-met, P53