Abstract: Objective: Analysis of the expression and function of mouse p38-2G4 protein in the differentiation of erythroid cells, and study whethere p38-2G4 is involved in the differentiation of erythroid. Methods The expression of p38-2G4 in fetal liver from E10.5 to E16.5, FVA cells and MEL cells induced by SB and HMBA were detected by Western bolt. And, the hemoglobin as a marker protein of erythroid cell differentiation was detected by benzidine staining. Results The expression of p38-2G4 in fetal liver cells of different differentiating stages increased at first and then decreased; the expression of p38-2G4 during FVA cells differentiating had a same trend; but it decreased during inducing. Conclusions Differential expression of p38-2G4 in different erythroid differentiation shows that p38-2G4 is involved in mouse erythroid differentiation.

Key words: p38-2G4, Fetal liver, FVA cell, inducing differentiation, erythroid differentiation