Abstract: Objective To detect MCPH1 mRNA expression in lung cancer, and over expression of MCPH1 in A-549 cells with drugs. Methods MCPH1 mRNA expression in lung cancer was measured by Real-time PCR.Eukaryotic expression plasmid pcDNA3.1(-)/MCPH1 and Blank plasmid pcDNA3.1(-) were transfected into A549 cells,and set three groups according to the different transfected plasmid: Experimental group (OVER)、Blank control group (W/O)、Untreated (NC). The MCPH1’S mRNA and protein expression were detected by quantitative Real-Time PCR and Western bolt. Results MCPH1 gene expression in cancer tissues is lower than normal tissues (P<0.05). In A549 cells，experimental groups were added respectively 1.0μmol/mL ADM, 20μg/mL TAX, 0.1μg/mL DDP ,48h later, The inhibition rate of experimental groups was significantly higher than the control groups and the untreated groups (P<0.05). Conclusion MCPH1 genes downregulated in lung cancer；the tumor inhibitory rate increased after MCPH1 gene overexpression concurrent chemotherapy drugs.

Key words: MCPH1, Real-time PCR, A549, Chemotherapeutic