Abstract: Objective: To observe the protective effect of adiponectin ( APN ) on high glucose induced vascular endothelial cell injury ,and investigate the expresstion of NF-κB and LOX-1 in the role of the protection process.Methods: D-Glucose of 30mmol/L effect on HUVEC-12 for 48 hours to make the model of injured vascular endothelial cells, adiponectin with different concentration (10,20,40μg/mL） for 48 hours and adiponectin of 20μg/mL for different time（12,24,48,72h）effected on the cell，inverted phase contrast microscope was used to observe the morphologic changes of the endothelial cells and the expression of NF-κB and LOX-1 protein in the HUVEC-12 was detected by Western Blotting, and NF-κB nuclear translocation in cells was observed by indirect immunofluorescence.Results: D-Glucose-induced HUVEC-12 were effected by adiponectin with different density and time, The morphologic changes of the cells converted to ameliorate: the reflected light of the HUVEC-12 was strengthened, particulate matter in cells tended to be not clear and the cell's shape converted from roundness to flat or polygon, NF-κB and LOX-1 protein expression were down-regulated partly with a dose and time-dependence（n=3,P<0.05）.Conclusion: Adiponectin (APN) may down-regulate LOX-1 protein expression by NF-kB signaling pathway so as to have the protective effect on D-Glucose-induced HUVEC-12.

Key words: D-Glucose, HUVEC-12, Adiponectin, LOX-1, NF-κB