Abstract: Objective　To observe the effects of 4,5,6,7-tetrabromobenzotriazole(TBB) on the expressions ofCK2ɑ，β-CATENIN and SURVIVINin thyroid papillary carcinoma cell line FTC133and to explore the possible underlyingmechanism. Methods FTC133 cellswere cultured in vitro and divided into control group(DMSO), TBBgroups(12.5, 25, 50μmol/L)randomly. The proliferationrateand migration of FTC133 cells was detected byMTTand wound healing method. TheCK2ɑ, β-CATENIN and SURVIVIN mRNA and protein expression levelswere determined by RT–PCR and Western blot. Results Compared with control group, the proliferationrate and migration of FTC133 cells wereinhibited by TBBin a dose–dependentmanner(P<0.01).The expression levels of mRNA and protein ofCK2ɑ were significantly decreased in the presence of different TBB in FTC133 cells（P<0.01）, the protein expression level of β-CATENIN and SURVIVIN were decreased, too（P<0.01）.Conclusion TBB inhibits the proliferation and migration of thyroid papillary carcinoma cell line FTC133 via WNT/β-CATENIN signaling pathway.

Key words: TBB, thyroid papillary carcinoma, CK2ɑ, β-CATENIN, SURVIVIN