Basic & Clinical Medicine ›› 2014, Vol. 34 ›› Issue (10): 1376-1380.
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Abstract: Objective To investigate the role of PTTG1 in the migration and invasion of Non-small cell lung cancer. Methods To detect the expression of PTTG1, E-cadherin and Vimentin in 80 cases of NSCLC using immunohistochemistry. Cells were divided by four groups : ①A549/con, cells were transfected by empty vector②A549/PTTG1, cells were transfected by purpose gene ③Scr/H1299, cells were transfected by empty vector ④SiPTTG1/H1299, cells were knocked down purpose gene. Western blot was used to analyze the expression of PTTG1, E-cadherin and Vimentin. Results The protein expression of PTTG1 in NSCLC was negatively correlated with E-cadherin and positively correlated with Vimentin (P<0.05). H1299 cells expressed higher PTTG1, and A549 cells expressed lower PTTG1. The expressions of PTTG1 and Vimentin were lower in SiPTTG1/H1299 cells than those in Scr/H1299 and H1299 cells after trancfected 24 hours, but the E-cadherin expression was higher. At the same time, the protein expression of Vimentin was up-regulated and the expression of E-cadherin was down-regulated in A549/PTTG1 cells. Conclusions PTTG1 can promote migration and invasion of NSCLC through EMT.
Key words: PTTG1, EMT, Lung tumor, migration
CLC Number:
R737.9
R73-37
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http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/Y2014/V34/I10/1376