Basic & Clinical Medicine ›› 2014, Vol. 34 ›› Issue (1): 62-67.
Previous Articles Next Articles
Received:
Revised:
Online:
Published:
Contact:
Abstract: Objective: To evaluate the contribution of the JAK2/STAT3 pathway to neuropathic pain,we examined the effects of this pathway in a rat model of bilateral chronic constriction injury (bCCI). Methods: A rat model of bCCI was established and 60 rats’ behavior tests were performed on the day before surgery and on day 3、7、14 and 21 after surgery, L4~L6 dorsal spinal cord was harvested at the each time point. RT-PCR and Western blot were performed to explore the activation of JAK2/STAT3 pathway. Results: Pain-related behavioral tests socres in the bCCI rats were significant decreased as compared to the sham-operated and na?ve group at each time point postoperatively (P<0.05). SOCS3 mRNA and STAT3 mRNA significantly increased on day 14, accompanied by JAK2mRNA of with a similar time course. IL-6 mRNA level increased on day 3 and showed statistically significant increases on day 21. Western blot analysis showed that JAK2, P-STAT3, SOCS3 increased at different timepoints. Conclusion:Our results suggest that the JAK2/STAT3 pathway in the spinal dorsal horn was significantly upregulated in a rat bCCI model of neuropathic pain which will open new avenues for therapeutic intervention.
Key words: Key words: JAK2, STAT3, neuropathic pain
CLC Number:
A
0 / / Recommend
Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks
URL: http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/
http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/Y2014/V34/I1/62