Abstract: Objective To investigate the effects of FK506 on cell proliferation of pancreatic NIT-1 beta-cell and insulin secretion function. Methods Effect of FK506 on proliferation and apoptotic were mensured by MTT-assay, Flowcytometry; Insulin secretion was investigated by Radioimmunoassay and Real-time PCR. Results FK506 (20μg?L-1) could inhibit the proliferation of NIT-1 cells, and could induce apoptosis; FK506(10μg?L-1) could inhibit NIT-1 cell insulin release, The mRNA expression of PDX-1 and GLUT2 were down-regulated. Conclusion FK506 inhibit NIT-1 cells insulin secretion, the mechanism maybe related to the down-regulation of. PDX-1 and GLUT2.

Key words: Key words: NIT-1 beta-cell, apoptosis, secretion level of insulin, PDX-1, GLUT2