Basic & Clinical Medicine ›› 2013, Vol. 33 ›› Issue (9): 1191-1194.
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Abstract: Objective To investigate the effects of FK506 on cell proliferation of pancreatic NIT-1 beta-cell and insulin secretion function. Methods Effect of FK506 on proliferation and apoptotic were mensured by MTT-assay, Flowcytometry; Insulin secretion was investigated by Radioimmunoassay and Real-time PCR. Results FK506 (20μg?L-1) could inhibit the proliferation of NIT-1 cells, and could induce apoptosis; FK506(10μg?L-1) could inhibit NIT-1 cell insulin release, The mRNA expression of PDX-1 and GLUT2 were down-regulated. Conclusion FK506 inhibit NIT-1 cells insulin secretion, the mechanism maybe related to the down-regulation of. PDX-1 and GLUT2.
Key words: Key words: NIT-1 beta-cell, apoptosis, secretion level of insulin, PDX-1, GLUT2
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URL: http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/
http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/Y2013/V33/I9/1191