Basic & Clinical Medicine ›› 2013, Vol. 33 ›› Issue (9): 1150-1154.
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Abstract: Objective To investigate the influence of heme oxygenase -1/carbon monoxide (HO-1/CO) system on apoptotic myocardial cells induced by angiotensin II (AngII) . Methods Primary culture of myocardial cells in neonatal Wistar rat, randomly divided cells into four groups. Respectively with Real Time - PCR and Western blot analyzed HO -1 mRNA and protein expression, colorimetric method was used for determination of cell culture supernatant fluid medium carbon oxygen hemoglobin content; flow cytometry instrument was used to detect cells apoptosis rate. Results AngII group of myocardial cell HO -1 mRNA, protein, COHb content and cell apoptosis were significantly higher than those in the control group (P < 0.05), AngII+hemin group HO -1 mRNA, protein and COHb content further raise (P < 0.05), while apoptosis rollback (P < 0.05), but still higher than those of the control group (P < 0.05), and AngII+ZnPPIX group only apoptosis significantly increased (P < 0.05), other index no significant change. Conclusion HO-1/CO system attenuate the myocardial cells apoptosis induced by AngII.
Key words: key words:heme oxygenase-1, Endogenous carbon monoxide, Myocardial cells, apoptosis
CLC Number:
R541.6
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URL: http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/
http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/Y2013/V33/I9/1150