Abstract: Objective Observe the effect of FGF2 in BMP9-induced osteogenic differentiation of C3H10T1/2 mesenchymal stem cells,and investigate its mechanism.Methods C3H10T1/2 cells were infected by adenovirus recombined with FGF2 and BMP9,then the early osteogenic maker alkaline phosphatase(ALP) activity were detected by quantitative and staining assay,later osteogenic maker calcium deposition was determined by Alizarin Red S staining,p12SBE-luc activity were detected by quantitative,Smad1/5/8,Runx2 and OCN were determined by western blot. Results BMP9-induced early osteogenic marker ALP, and late osteogenic markers matrix mineralization and osteocalcin (OCN) are inhibited by FGF2, BMP-Smad1/5/8 pathway and Runx2 are inhibited by FGF2.Conclusion FGF2 inhibits bone morphogenetic protein9-induced osteogenic differentiation of C3H10T1/2 mesenchymal stem cells.

Key words: Key words :FGF2 , BMP9, mesenchymal stem cells , osteogenic differentiation