Basic & Clinical Medicine ›› 2013, Vol. 33 ›› Issue (8): 1043-1049.

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HGF and G-CSF induce the differentiation of rat bone marrow mesenchymal stem cells into hepatocyte-like Cells


  • Received:2012-09-28 Revised:2012-12-07 Online:2013-08-05 Published:2013-07-18

Abstract: Objective To investigate if the hepatocyte growth factor(HGF)and granlocyte-colony stimulating factor (G-CSF) could induce differentiation of rat bone marrow mesenchymal stem cells(BM-MSCs)into hepatocyte-like cells .Methods The BM-MSCs were isolated and cultured in- vitro by adherent method,then identified by flow cytometry.The BM-MSCs of the third passage were used as four groups - control group(10%FBS)and intervation groups(0.1μmol/L G-CSF;20ng/mLHGF;0.1μmol/L G-CSF+20ng/mLHGF).The mRNA and protein were extracted on 7,14,21 days,then detected by RT-PCR and Western-blot. Results.The third passage BM-MSCs of intervation groups (20ng/mLHGF and 0.1μmol/L G-CSF+20ng/mLHGF) got hepatocyte like feafure after induction.The results tested by Flow cytometry analysis were as follows: CD34-PE0.3%,CD45-FITC0.1%,CD90-FITC99.6%,CD105-PE99.8%. The mRNA and protein of ALB and AFP were detected respetively by RT-PCR and Western-blot on day 7,14,and 21 from inervation groups (10%FBS+20ng/mLHGF;10%FBS+0.1μmol/L G-CSF+20ng/mLHGF), however ,the amount of ALB increased as time went by ,while AFP decreased to extremely low on day 21.Meaningfully,the difference between the two groups is significant(P<0.05). Conclusion HGF can induce the differentiation of BM-MSCs into hepatocyte-like cells, which can be significantly enhanced by G-CSF.

Key words: 【Keywords】Bone marrow mesenchymal stem cells, Hepatocyte growth factor, granlocyte-colony stimulating factor, Hepatocyte-like cells