Basic & Clinical Medicine ›› 2013, Vol. 33 ›› Issue (8): 1014-1018.

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Bone morphogenetic protein 4 affects angiogenesis in vitro of human umbilical cord vein endothelial cells through ERK/MAPK pathway

Qin WANG,1,ZHANG Hong-gang 3, ZHANG Qiu-ju 3,Rui-juan XIU   

  • Received:2012-09-10 Revised:2012-11-08 Online:2013-08-05 Published:2013-07-18
  • Contact: ZHANG Hong-gang

Abstract: Objective To observe the effects of BMP-4 on HUVECs proliferation, migration and capillary sprouting, and signal pathway involved in these biological behaviors. Methods HUVECs were treated with BMP-4. HUVEC proliferation was determined by BrdU incorporation, wound-healing was applied to observe HUVEC migration. Cell lysates were analysed by Western blotting for ERK1/2 phosphorylation. Results Treatment with BMP-4 results in dose dependent inhibition of HUVEC proliferation, migration and capillary sprouting at lower concentrations(5、10 and 20 ng/mL), but at higher dose of BMP-4(50 and 100 ng/mL), this inhibition disappeared. Conclusions HUVEC proliferation , migration and capillary sprouting induced by BMP-4 might be dependent on ERK phosphorylation.

Key words: bone morphogenetic protein, human umbilical vein endothelial cell, ERK1/2

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