Abstract: Objective To choose the optimum conditions for inducing mouse reticulocytes by using hytrazinobenzene hydrochloride. Methods One percent hytrazinobenzene hydrochloride was injected to back subcutaneous tissue of the ICR and BALB/c mouse everyday. The dose was100 μl, 200 μl, 300 μl and 400 μl, respectively for 9 days. Then tail vein blood was taken every day, and observed. After staining the reticulocyte induced efficiency was statistical analyzed by using the bright tar blue staining. Results The morphology and proportion of reticulocytes were the best when the mice were injected 200 μl hytrazinobenzene hydrochloride for 4 days. The ratio of induced reticulocyte was about 83.16% ±3.41%. The cell shape of reticulocytes was irregular, the state of cells was poor and animals began to die as well when mice were injected 300 μl or 400 μl hytrazinobenzene hydrochloride. Conclusions The best conditions for inducing reticulocyte are as follows: weight 25 to 35 g mice daily injection of 200 μl 1% hytrazinobenzene hydrochloride for 4 days.

Key words: reticulocyte, hytrazinobenzene hydrochloride, induced conditions