Abstract: Object To investigate the effect of the drug-containing serum of Liu Wei Di Huang Wan on PC12 cells which were damaged by KCl. Method We established the PC12 cell models induced by KCl which were divided into three groups: control group, model group, Liu Wei Di Huang Wan containing serum group. The morphology of cells in each group were observed with inverted phase contrast microscope. The proliferation of PC12 cells was detected by MTT assay and the concentration of LDH in PC12 cells were analysed. The level of CREB mRNA was analysed by RT-PCR. The expression of CREB was analysed by SABC-immunohistochemistry stain. Result The activity of LDH released from cells was increased and the level of CREB was decreased in the model group （P<0.01）compared with the control group. It significantly improved the activity of cells and the level of CREB mRNA in Liu Wei Di Huang Wan serum group compared with the model group （P<0.01）. Conclusion It can reduce the damage of PC12 cells induced by KCl by the drug-containing serum with Liu Wei Di Huang Wan.

Key words: Liu Wei Di Huang Wan, PC12 cell, KCl,, CREB