Abstract: Objective To introduce a serum oxidized α1-antitrypsin (Ox-AT) quantitative detection kit, and analyze serum Ox-AT in Chinese male smokers. Method Set up a sandwich ELISA method with Ox-AT and a specific anti-Ox-AT monoclonal antibody. Analyze serum Ox-AT level of 20 pairs of smoking and non-smoking male. Results Ox-AT sELISA has a good linear in range of 0-500μg/L. The limit detection is 2.58μg/L, CV among batches is 11.27%, the accuracy is about 88.19%, the shelf life under 4℃ is more than 6 months. Detecting with this sELISA, serum Ox-AT level in smoking male is 55.43 ± 49.94μg/L, non-smoking control group is 11.01 ± 12.15μg/L, p <0.001. Conclusion The Ox-AT detection kit can be used for quantitative testing serum Ox-AT. Ox-AT might be more useful than α1-AT in evaluating Chinese COPD.

Key words: α1-antitrypsin, ELISA, Chronic obstructive pulmonary disease