Basic & Clinical Medicine ›› 2013, Vol. 33 ›› Issue (5): 588-592.
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Cheng-Gang LIU1,
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Abstract: Objective To investigate the function and mechanism of miR-184 on cornea neovascularization induced by alkali burning. Methods cornea neovascularization(CRNV) was induced by alkali burning. HUVEC was cultured on Three-dimensional Matrigel. qRT-PCR was performed to analyze microRNA-184 expression in vivo and in vitro. Predicted and analyzed the target genes of microRNA-184 by bioinformatics method. Results Delvelop a model for angiogenesis both in vivo and in vitro. The expression of miR-184 on CRNV (0.145±0.013) was sigificantly less than transparent cornea (1.015±0.189) (P <0.01), and was just absolutely miniscule on HUVEC(0.007±0.004 ) (P <0.05). The targets of miR-184 predicted was associated with VEGF singaling pathways. Conclusion The marked decrease of miR-184 expression during angiogenesis and target prediction suggest a potential role and mechanism of miR-184 played by VEGF singaling pathways on CRNV.
Key words: cornea neovascularization, three-dimensional culture, microRNA, target gene prediction
CLC Number:
R 772.2
Cheng-Gang LIU. The expression of miR-184 on the alkali burning induced rat cornea neovascularization and the target genes analysis[J]. Basic & Clinical Medicine, 2013, 33(5): 588-592.
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http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/Y2013/V33/I5/588