Basic & Clinical Medicine ›› 2013, Vol. 33 ›› Issue (5): 588-592.

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The expression of miR-184 on the alkali burning induced rat cornea neovascularization and the target genes analysis

Cheng-Gang LIU1,   

  • Received:2012-03-31 Revised:2012-10-26 Online:2013-05-05 Published:2013-05-29

Abstract: Objective To investigate the function and mechanism of miR-184 on cornea neovascularization induced by alkali burning. Methods cornea neovascularization(CRNV) was induced by alkali burning. HUVEC was cultured on Three-dimensional Matrigel. qRT-PCR was performed to analyze microRNA-184 expression in vivo and in vitro. Predicted and analyzed the target genes of microRNA-184 by bioinformatics method. Results Delvelop a model for angiogenesis both in vivo and in vitro. The expression of miR-184 on CRNV (0.145±0.013) was sigificantly less than transparent cornea (1.015±0.189) (P <0.01), and was just absolutely miniscule on HUVEC(0.007±0.004 ) (P <0.05). The targets of miR-184 predicted was associated with VEGF singaling pathways. Conclusion The marked decrease of miR-184 expression during angiogenesis and target prediction suggest a potential role and mechanism of miR-184 played by VEGF singaling pathways on CRNV.

Key words: cornea neovascularization, three-dimensional culture, microRNA, target gene prediction

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