Abstract: Objective To explore the expression and distribution of H2-Calponin mRNA and protein in four cancerous tissues and its clinical significance. Methods In situ RT- PCR was used to detect the expression and distribution of H2-Calponin mRNA in four different cancerous tissues. Immunohistochemistry was used to detect the expression of H2-Calponin protein in lung cancer, gastric carcinoma, nasopharyngeal carcinoma(NPC) and hepatocellular carcinoma(HCC). Results H2-Calponin protein was mainly expressed in cytoplasm by immunohistochemistry.The positive rates of H2-calponin protein in lung cancer, gastric carcinoma,nasopharyngeal carcinoma and HCC were 30.0%,27.5%,45.0% and 51.8%,respectively.The expression positive rate of H2-Calponin in HCC was the highest. The expression of H2-Calponin mRNA was showed near the nucleus by in situ RT- PCR.The mRNA expression rates of H2-Calponin in lung cancer,gastric carcinoma,nasopharyngeal carcinoma and HCC were 17.5%,10.0%,20.0% and 50.0%,respectively.The positive rate of H2-Calponin in HCC was the highest(P<0.05). The positive rate of H2-calponin protein in HCC with metastasis was higher than that without metastasis.The expression of H2-Calponin protein in four different cancerous tissues had no statistically significant with patient gender,age and pathologic differentiation grade. Conclusions H2-Calponin protein is a correlated with HCC, specially positive correlated with HCC with metastasis.

Key words: H2-Calponin, hepatocarcinoma, immunohistochemistry, in situ RT- PCR