Abstract: Objective The purpose of this study is to study the increased effects of curcumin (CUR) on A549 cell proliferation inhibition and induction apoptosis via including CUR into the hydroxypropyl beta cyclodextrin inclusion. Methods The inhibition rates were determined by MTT assay. Invert microscope was used to observe the changes of A549 cells after reated with CUR and CUR Hydroxypropyl beta cyclodextrin(CURCD) . Flow cytometric analysis was performed to detecte the cell cycle, apoptosis, reactive oxygen species, free Ca2+ concentration, mitochondrial membrane potential. Results CURCD had effects on the proliferative activity of human pulmonary adenocarcinoma cell line A549 in a dose-dependent manner. Flow cytometric analysis indicated that cell cycles was blockaged at S/G2 phases, and cell apoptosis was obviously observed, reactive oxygen species and free Ca2+ concentration were increased, mitochondrial membrane potential were decreased. The differences were remarkable between CUR and CURCD. Conclusion CURCD obviously increases the inhibition effects of CUR on A549 cell proliferation and induces its apoptosis.

Key words: Curcumin, Hydroxypropyl beta cyclodextrin, A549 cell, Apoptosis