Abstract: Objective Anti-apoptotosis and antioxidant effect of luteolin pretreatment on neonatal rat myocardial cells induced by hydrogen peroxide. Methods Myocardial cells are clipped from 1-3 days of neonatal rat. Different groups were set after conventional culture,such as normal groups, the model groups,1μmol/L、10μmol/L、50μmol/L、100μmol/L and 200μmol/L luteolin pretreatment groups. After pretreatment two hours and the 1mmol/L H2O2 oxidative injury 2 hours, MTT datas are detected each group. We selected 100μmol/L luteolin as the pretreatment groups of baseing on the results of the MTT. The different groups are observed about cell morphology, cell pulsating, Hoechst33342-PI double-staining and detected MMP by Rhodamine 123 staining. The activity is detected of LDH,MDA and SOD，and detection of apoptosis . Results Compared with model groups, luteolin pretreatment groups of myocardial cell viability is significantly（P<0.01）. The morphology and pulsating are good condition（P<0.01）and apoptosis reduction, and the degree of injury of the mitochondrial is decreased（P<0.01） and SOD activity increased（P<0.01）, LDH and MDA generation is suppressed（P<0.01）. Conclusion Preconditioning of luteolin on H2O2-induced myocardial injury has a protective effect. Anti-apoptotic effect of luteolin may be related to the antioxidant.

Key words: luteolin, myocardial cell, hydrogen peroxide induced, Anti-apoptotic, antioxidant.