Abstract: Objective To analyze the serum creatinine level among apparently healthy adolescents in Heilongjiang Province and establish the reference intervals of serum creatinine in different gender and different age groups of adolescents. Methods Large sample clinical epidemiological investigation was applied by two-stage clustering sampling method. Random sample of 2 281 primary and secondary school students from 8 to 18-year-old was selected from different district of Heilongjiang Province including Harbin and Mudanjiang from July 2008 to June 2009. The fasting venous blood was collected and serum was separated, then serum creatinine concentration was measured as soon as possible. After screening outlier individual, a total of 2 276 subjects were enrolled involving 1 039 male and 1 237 female, of which 1 392 Han and 884 Chinese Korean. Percentile was used to describe the distribution of serum Cr level of different growth stages and the reference intervals of serum Cr for adolescents were established by gender and age (P2.5 to P97.5). Results The reference intervals of creatinine for <11, 11～12, 13～14, 15～16, and 17～18 year-old males were 35～60、39～70、45～81、54～88、60～98μmol/L respectively while for females were 35～60、39～70、45～81、54～88、60～98μmol/L respectively. The concentration of Cr increased with age and was higher in Males than in Females(P<0.001). No difference of Cr level was seen between Chinese Han and Chinese Korean. Conclusion The reference intervals of serum creatinine for healthy adolescents in Heilongjiang Province are established, which supply good reference value for clinicians, especially pediatricians to judge and assess renal function in patients 8 to 18 years of age．