Abstract: Abstract: Objective Define the infection efficiency of 2009 novel swine-origin influenza A virus and seasonal influenza virus in human airway epithelial cells. Methods Infection efficiency of H1N1 virus in A549, CNE-2Z, H1650, Hep-2 and MRC-5 was examined by indirect immunofluorescence assay. Effect of inhibitors in human airway epithelial cells infected by H1N1 virus was examined by indirect immunofluorescence assay. Results （1）Infection efficiency of swine-origin H1N1 virus: H1650>A549>CNE-2Z>MRC-5>Hep-2；Infection efficiency of seasonal H1N1 virus: A549>CNE2Z>H1650>MRC-5>Hep-2;（2）Swine-origin H1N1 and seasonal H1N1 both entered into airway epithelial cells by the way of endocytosis. Conclusion Infection efficiency of seasonal H1N1 virus was clearly higher than swine-origin H1N1 virus in A549 cells; Infection efficiency of swine-origin H1N1 virus was clearly higher than seasonal H1N1 virus in H1650 cells as infection time increased.

Key words: Key words: S-OIV H1N1, influenza virus, airway epithelial cells, infection efficiency