Abstract: Abstract：Objective To study the regulation of Erythroid Kruppel-like factor (ELKF) on the expression of miR-96 in Erythropoiesis. Method K562 cells were induced to erythroid differentiation by Hemin. The expression of miR-96 during erythroid differentiation was detected by real-time PCR. Bioinformatic analysis was used to search for binding sites for EKLF within 2kb upstream sequence of transcriptional start site (TSS) of miR-96. Biology function of these sites were detected by ChIP-PCR combined with Dual-Luciferase Reporter Assay. Result miR-96 expression was increased during erythroid differentiation of k562 cells induced by Hemin and peaked at 48h (3.94156±0.63995, p<0.05). Several binding sites within 2kb upstream sequence of TSS of miR-96, occupied with EKLF can recruit RNA polymerase II (Pol II) and promote the expression of downstream genes. Conclusion EKLF activates the expression of miR-96 in Erythropoiesis directly.

Key words: Erythroid differentiation, EKLF, miR-96, ChIP