Abstract: Objective: To establish a method of efficient production of hair like cells from bone marrow mesenchymal stem cells（MSCs）in vitro. Methods: MSCs、astrocytes(ASTs) and basement membrane cells (BAMCs) were isolated from mice, passed and depurated into third generation.We co-cultured MSCs with astrocytes(ASTs) in DMEM/F12/RA+10%FBS for 7 days, then checked the surface molecule of Nestin by immunocytochemical analyses.Afterward,These cells were co-cultured with BAMCs for 14 days in DMEM/F12+10%FBS.Then checked the expression of hair cell markers(MyosinVIIa and Math1 )by immunocytochemical analyses.We checked the mRNA expression of hair cell markers（MyosinVIIa and Math1）by RT-PCR.Results: MSCs which were co-cultured with ASTs for 7 days could express Nestin.Then Nestin-positive cells co-cultured with BAMCs for 14 days could express mRNA and protein of hair cell markers（Math1 and MyosinVIIa）.Conclusions: MSCs co-cultured with ASTs and BAMCs could be induced to differentiate into hair like cells.

Key words: mesenchymal stem cells, hair cells, co-cultured