Abstract: Object To analyze the relationship between sialylated glycopeptides from haptoglobin-beta chain in plasma at different treat time points and prognosis of patients with leukemia. Methods Plasma glycoproteins collected different treat time points, of the eight patients were enriched using lectin and then haptoglobin-beta chain were separated by gel. Resulting proteins were digested using trypsin, followed by proteinase K in gel, and the glycopeptides were enriched using liquid chromatography, followed by the structural characterization using tandem mass spectrometry. The change trends of sialylation were also analyzed. Results A decrease in the intensity of the sialylated glycopeptides of haptoglobin-beta chain was observed with disease recovery. Conclusion The changes in the level of the sialylated glycopeptides of haptoglobin were related to the prognosis of patients with leukemia. These sialylated glycopeptides may be a novel marker for monitoring the prognosis of leukemia.

Key words: Haptoglobin-beta chain, Sialylation, leukemia