Abstract: Abstract: Objective To compare the structural similarities of human secretory phospholipase A2 (sPLA2) with snake venom sPLA2, and to analyze the neutralization effect of sPLA2 inhibitors (PLIs) from snake serum against sPLA2 by exploring their structure and function relationship, which was meaningful for assessing PLIs’ clinical application in inflammation protection. Methods Multiple sequencial alignment of 7 human sPLA2 and 3 five-pace snake venom sPLA2 was made by Clustal W. Tertiary structures of the 10 sPLA2 were predicted by Swiss-Modelling. Spatial structure similarity between snake venom and human sPLA2 was assessed by UCSF Chiema. Results Human and snake venom sPLA2 shared 40% sequencial homologous and approximately similar spatial structure. All the PLIs (γ type) have similar tertiary charectristics and conserved binding region是 to sPLA2. Conclusion Based on the bioinformatical analysis, snake serum PLIs were considered as an effective inhibitor to snake venom toxicity and human sPLA2 activity. Thus, the PLIs were potential special blockers to human sPLA2 inflammation pathway and meaningful for curing clinical disease.

Key words: phospholipase A2, phospholipase A2 inhibitor, bioinformatics