Abstract: Abstract:Objective By investigating effect of the dynamic expression of HSP47 in human embryonic lung fibroblast (HELF) on pulmonary fibrosis to explore the relation between HSP47 and pulmonary fibrosis . Methods Dynamic expression of HSP47、COLLAGENⅠ、α-SMA and VIMENTIN were observed after HSP47 was induced to expressed in HELF in incubation of TGF-β1. HELF were stimulated by TGF-β1(5ng/mL) at different time points and different dosages for 48h respectively. The proliferative rate of HELF was tested by the method ofWST.Expression of HSP47 was determined by immunofluorescence.Expressions of HSP47、COLLAGEⅠ、α-SMA and VIMENTIN were detected by Western blot. Results HSP47 showed slight expression in normal HELF. Expressions of HSP47, COLLAGENⅠ,α-SMA and VIMENTIN increased synchronously by means of time-independence and dosage-independence after HELF being treated by TGF-β1（P<0.05）.Conclusions HSP47 participated in the expression of collagen in human embryonic lung fibroblast induced by TGF－β1 that suggested HSP47 might play an important role in pulmonary fibrosis as collagen-specific molecular chaperone.