Abstract: Objective To explore the characteristic of bone remodeling by establishing type 2 diabetic osteoporosis rat model. Methods Female Wistar rats were randomly divided into sham operation group (NS); bilateral ovariectomy group (NOVX); diabetic sham operation group (DS ) and diabetic ovariectomized group (DOVX ). Ovariectomy was performed in both control and diabetic rats after type 2 diabetic models had been made . Venous blood samples were collected at 0, 4, 8 and 12wks after ovariectomy，fasting plasma glucose(FPG)、fasting insulin(FINS)、estrogen(E2) (B-ALP),tartrate-resistant acid phosphatase (TRACP5b) were all measured. BMD were measured , osteoblast and osteoclast cell were isolated and cultured from rats of all groups. Results At 0wks，FPG had significantly increased when DOVX' 13.1±4.9 mmol/L compared with NS’4.4±0.6mmol/L（all P<0.05）. at 4wks, BMD had significantly decreased when DOVX'0.073±0.012g/cm2 compared with DS'0.098±0.016g/cm2（P<0.05）.at 4、8 and 12wks, there were no differences in plasma B-ALP levels when DOVX rats compared with NS rats.at 4 and 8wks,compared with NS rats, DOVX rats had significantly higher plasma TRACP5b concentration (all p<0.05). For the activity of Osteoblast , there were no differences when DOVX compared with NS , NOVX and DS group . osteoclast numbers were higher in DOVX rats than those from NS rats(P <0.05).Conclusion There were actived bone resorption and relatively slow bone formation in type 2 diabetic osteoporosis rats.

Key words: Type 2 diabetes, ovariectomy, osteoblast, osteoclast, bone remodeling