Basic & Clinical Medicine ›› 2011, Vol. 31 ›› Issue (6): 683-687.

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The activation of P38MAPK signaling pathway increases intracellular Aβ production in SH-SY5Y

Xue-wen GUO,Liang LI   

  1. Capital Medical University
  • Received:2010-12-10 Revised:2011-04-08 Online:2011-06-05 Published:2011-06-06
  • Contact: Liang LI E-mail:liliang@ccmu.edu.cn
  • Supported by:
    Natural Science Foundation of Beijing

Abstract: Objective To investigate the effect of the activation of P38MAPK signaling pathway on the intracellular β-amyloid(Aβ) production in human neuroblastoma SH-SY5Y. Methods SH-SY5Y cells were used to test intracellular Aβ levels by ELISA after activation of P38MAPK signaling pathway by anisomycin for 1 and 72 h.The mRNA and protein levels of APP, PS1 and BACE1 were examined by real time quantitative PCR and Western blot respectively. Results The expression of Phospho-P38MAPK was increased after anisomycin treatment 1and 72 h; the production of Aβ1-40 was about (46.88±3.60) pg/mL[p<0.05,Con group was (39.09±1.60) pg/mL]after anisomycin treatment for 72h; APP and PS1 mRNA was increased after anisomycin treatment for 1h, while APP, PS1 and BACE1 mRNA was significantly increased after anisomycin treatment for 72h;the protein levels of APP, PS1 and BACE1 were increased as 1.45-fold,1.38-fold and 1.60-fold respectively as control after anisomycin treatment for 72h while nothing alter for 1h. Conclusion Activation of P38MAPK signaling pathway could increase Aβ production and play a role in the pathygensis of Alzheimer's disease(AD).

Key words: Alzheimer's disease, p38MAPK, β-Amyloid(Aβ).

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