Basic & Clinical Medicine ›› 2011, Vol. 31 ›› Issue (6): 667-671.

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DNA vaccine encoding Ebola virus envelope glycoprotein inducing high-titer neutralization antibody in mice

Gui-gen ZHANG1,Jian-she LANG2,Hong-liang WANG2,Kang-tai LIU2,Cheng-yu JIANG1   

  1. 1. Institute of Basic Medical Sciences, CAMS & PUMC
  • Received:2011-03-22 Revised:2011-03-30 Online:2011-06-05 Published:2011-06-06
  • Contact: Cheng-yu JIANG
  • Supported by:
    National Natural Science Foundation of China

Abstract: Obejective To study the characteristics of neutralization antibody in mice induced by DNA vaccine encoding Zaire ebolavirus glycoprotein (GP) and develop DNA vaccine against Ebola virus. Methods Zaire Ebolavirus GP encoding plasmid Peak13CD5LGP was constructed. 100 ?g dosage of Peak13CD5LGP was used to immunize the mice, the titer of neutralization antibody against Ebola Zaire virus glycoprotein was assayed by ELISA using Ebola Zaire virus glycoprotein subunit GP1-Fc fusion protien as capture antigen. Results After boost immunization with 100 ?g dosage Peak13CD5LGP plasmid, the titer of neutralization antibody of anti-GP was 1:2000, and both efficiency and specificity of neutralization antibody was verified by western blot. Conlusion 100?g dosage of DNA vaccine encoding Zaire Ebolavirus envelope glycoprotein is able to induce persistent and high level of neutralizing anibody, and may be potential valuable vaccine against the deadly Zaire Ebolavirus.

Key words: Zaire Ebolavirus, envelope glycoprotein, DNA vaccine

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